Transplantation, Immunology and Inflammation T2i - EA 4245 - Tours
- Dr Sébastien ROGER
Research axis :
- Physiopathology and prevention of tissue lesions induced by ischemia in cardiac infarction, in solid tumours and in organ transplantation. Study of cell-to-cell communications, cell and molecular signalling pathways involved.
- Study of the immuno-inflammatory response during ischemia reperfusion.
- Identification of soluble and membrane biomarkers of organ dysfunction (tissue injury, rejection, relapse).
- Development and follow-up of new therapeutic strategies (antibodies and pharmacological agents) to prevent tissue damage following ischemia-reperfusion or tissue hypoxia.
Expertise / know-how:
- Molecular Biology (RT-qPCR, western blotting), ELISA, cell phenotyping and cell sorting, cell imaging, anatomopathology
- Analysis of the activity of ion channels and ionotropic receptors (cell electrophysiology, spectrofluorimetry)
- Analysis of the immune-inflammatory response under normal conditions or hypoxia / reoxygenation : proliferation, viability, cytokines, adhesion/migration, multi-staining by flow cytometry, cytotoxicity…
- Culture of cancer cells (mammary and colon epithelial origin) ; Establishment of primary colorectal cultures from biopsies ; Differentiation of human monocytes into dendritic cells ; Culture of cardiomyocytes and cardiac fibroblasts, renal tubular proximal cells.
- Immortalization by EBV technique and culture of human B lymphocytes
- Biological system modeling
- Pharmacokinetics and pharmacodynamics applied to therapeutic antibodies
1st and 2nd floor; Vialle building, faculty of Medicine, University of Tours, France
BCR Facility level -1 Bretonneau building, faculty Medicine